Journal: Research Square

Article Title: Lineage specific extracellular vesicle-associated protein biomarkers for the early detection of high grade serous ovarian cancer

doi: 10.21203/rs.3.rs-2814022/v1

Figure Lengend Snippet: Enrichment and characterization of cell line and tissue explant EVs. a) Surgical resections of healthy FT or tumor tissues were minced and used to initiate short-term tissue explants (cultured for 24 h) followed by collection of the conditioned media and processed by differential ultracentrifugation to enrich for EVs. Likewise, conditioned media from the FT and ovarian cancer cell lines shown was collected and processed. Created with BioRender.com b) Representative NTA data. c) Sixty (60) EV particles were imaged, and their size was measured for representative samples by TEM at x30K magnification. d) Shown are representative SP-IRIS and fluorescence (ExoView) data of FT tissue explant derived EVs displaying the common tetraspanins observed with cell-line derived EVs: CD9 (blue), CD63 (pink) and CD81 (green).

Article Snippet: For NTA data and IHC expression score analysis, GraphPad Prism version 8 was used for performing unpaired Mann-Whitney t-tests and calculating p-values to compare between the samples.

Techniques: Cell Culture, Fluorescence, Derivative Assay

Journal: Cancers

Article Title: How Should We Test for Lynch Syndrome? A Review of Current Guidelines and Future Strategies

doi: 10.3390/cancers13030406

Figure Lengend Snippet: Estimated frequency of Lynch syndrome (LS) among unselected patients of different Lynch-spectrum, and other, tumour types. Unselected means no selection of patient by clinical features, such as age or family history. However, some studies selected patients for germline MMR gene testing by tumour MMR deficiency testing.

Article Snippet: , 13/13 (100.0%)/10/10 (100.0%) , MSI by FLA of 2 monoNRs and 3 diNRs (Bethesda panel)/IHC of MLH1, MSH2, and MSH6 , Gylling et al., 2008 [ ] .

Techniques: Selection, Methylation, Biomarker Discovery

Journal: Cancers

Article Title: How Should We Test for Lynch Syndrome? A Review of Current Guidelines and Future Strategies

doi: 10.3390/cancers13030406

Figure Lengend Snippet: Estimated frequency of mismatch repair (MMR) deficiency in different tumour types from Lynch syndrome (LS) gene carriers. Studies were only included if the LS diagnosis was made independent of tumour MMR status.

Article Snippet: , 13/13 (100.0%)/10/10 (100.0%) , MSI by FLA of 2 monoNRs and 3 diNRs (Bethesda panel)/IHC of MLH1, MSH2, and MSH6 , Gylling et al., 2008 [ ] .

Techniques: Biomarker Discovery

Journal: Cancers

Article Title: How Should We Test for Lynch Syndrome? A Review of Current Guidelines and Future Strategies

doi: 10.3390/cancers13030406

Figure Lengend Snippet: Estimated frequency of mismatch repair (MMR) deficiency among unselected tumours of different Lynch-spectrum, and other, tumour types. Unselected means no selection of tumours by the patients’ clinical features, such as age or family history.

Article Snippet: , 13/13 (100.0%)/10/10 (100.0%) , MSI by FLA of 2 monoNRs and 3 diNRs (Bethesda panel)/IHC of MLH1, MSH2, and MSH6 , Gylling et al., 2008 [ ] .

Techniques: Selection, Southern Blot, Marker